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Physiological and biochemical features of some cultivars in essential oil rose (Rosa x damascena Mill.) growing in situ and in vitro
(2016)  Page(s) 226-232.  
 
The study involved some valuable essential oil rose cultivars, growing on the collection plots of NBG-NSC [Nikita Botanical Garden - National Science Centre]: Raduga, Festivalnaya, Lany, Michurinka.
....The level of water deficit in the first decade of June (average air temperature 21.9°C, minimum relative humidity – 41%) ranged from 15 to 18% in the cultivars Raduga, Festivalnaya and Michurinka; this index was higher in the cultivar Lany (23%). Data obtained in the process of diagnosing the functional state of the leaves through the fluorescence induction of the exposed leaf, demonstrated the absence of photosynthetic apparatus disturbances (relative photosynthetic activity in field studies ranged from 0.66 (‘Michurinka’) to 0.79 a.u. (‘Festivalnaya’). The water holding capacity of leaf tissue was the highest in cultivars Festivalnaya (after 8 hours leaves lose 24-26% of water, after 24 hours – 44-48%) and ‘Raduga’ (28-34% and 39-50%). Minimum water holding capacity was in the cultivars Lany and Michurinka.
....Rapid water loss under wilting of exposed leaves occurred after 4 hours in cultivars Michurinka (21%) and Lany (18%), and after 6 hours of wilting in the cultivars Raduga and Festivalnaya to the level of 20-23%. During the growing season we also observed increase of water holding capacity of leaf tissues: within 24 hours the water loss was 42-48%. Under significant water loss (25-30%) in the leaves of the cultivar Festivalnaya inhibition of photosynthetic function ((Fm-Fst)/Fm=0.75 a.u.) wasn`t observed. In the cultivar Michurinka leaves under the water loss of 15% disturbances in photosystem-2 ((Fm-Fst)/Fm=0.54 a.u.) and at the level of lightharvesting complexes of the photosystem inhibition was noted after 8 hours wilting (water loss 35%).
Thus, on the base of essential oil rose water regime analyses in situ cultivars Festivalnaya and Raduga could be marked as plants with higher ecological and physiological potential in arid conditions.
....Concentration of ascorbic acid, depending on the cultivar, varied in the range of 24.99 mg/100 g (‘Michurinka’) up to 47.87 mg/100 g (‘Lany’). Proline content in studied rose cultivars ranged from 9.42mg/100 g (‘Festivalnaya’) to 12.95 mg/100 g (‘Michurinka’).
During vegetation period under hydrothermal stress the total content of phenolic compounds,
particularly flavanols, increased in studied plants, except the cultivar Michurinka. Despite the slower
metabolism of phenolic compounds in the absence of intensive plants` growth and development, increased
synthesis of these compounds may correspond to plant response over stress caused by overheating.
Concentrations of ascorbic acid and proline in this vegetation period varied ambiguous: in the cultivars Festivalnaya and Raduga proline content decreased, whereas ascorbic acid – increased. In the cultivars Lany and Michurinka the opposite trend was noted. The reasons for these differences are specific reaction of plants to the temperature stress, i.e. primary accumulation of specific protective compounds, and physiological features of plant development in this period.
....Phenolic compounds` content in plants cultured in vitro was quite high (1056-1958 mg/100 g). The increased accumulation of phenolic compounds demonstrated high adaptability to the growth conditions and resistance to unfavourable environmental conditions. Thus flavonol concentration in plants cultured in vitro was significantly lower than in intact plants – 23.4-52.0 mg/100g (maximum in ‘Festivalnaya’). Ascorbic acidconcentration in plants in vitro was lower than in intact plants have not been subjected to overheating.
On the contrary, proline content in vitro was higher than in intact plants have not been subjected to thermal stress, up to 10.99-19.62 mg/100 g (maximum in ‘Raduga’). High amount of this compound isprobably, caused with plants` growth and metabolism, as well as nutrients, vitamins and growth regulators level in the culture medium.
Maximum rates of catalase, superoxide dismutase and polyphenoloxidase activity were found in rose cultivars Festivalnaya, Lany, Raduga, cultured in vitro (Table 4).
 
(2016)  Page(s) 226-232.  
 
The study involved some valuable essential oil rose cultivars, growing on the collection plots of NBG-NSC [Nikita Botanical Garden - National Science Centre]: Raduga, Festivalnaya, Lany, Michurinka.
....The level of water deficit in the first decade of June (average air temperature 21.9°C, minimum relative humidity – 41%) ranged from 15 to 18% in the cultivars Raduga, Festivalnaya and Michurinka; this index was higher in the cultivar Lany (23%). Data obtained in the process of diagnosing the functional state of the leaves through the fluorescence induction of the exposed leaf, demonstrated the absence of photosynthetic apparatus disturbances (relative photosynthetic activity in field studies ranged from 0.66 (‘Michurinka’) to 0.79 a.u. (‘Festivalnaya’). The water holding capacity of leaf tissue was the highest in cultivars Festivalnaya (after 8 hours leaves lose 24-26% of water, after 24 hours – 44-48%) and ‘Raduga’ (28-34% and 39-50%). Minimum water holding capacity was in the cultivars Lany and Michurinka.
....Rapid water loss under wilting of exposed leaves occurred after 4 hours in cultivars Michurinka (21%) and Lany (18%), and after 6 hours of wilting in the cultivars Raduga and Festivalnaya to the level of 20-23%. During the growing season we also observed increase of water holding capacity of leaf tissues: within 24 hours the water loss was 42-48%. Under significant water loss (25-30%) in the leaves of the cultivar Festivalnaya inhibition of photosynthetic function ((Fm-Fst)/Fm=0.75 a.u.) wasn`t observed. In the cultivar Michurinka leaves under the water loss of 15% disturbances in photosystem-2 ((Fm-Fst)/Fm=0.54 a.u.) and at the level of lightharvesting complexes of the photosystem inhibition was noted after 8 hours wilting (water loss 35%).
Thus, on the base of essential oil rose water regime analyses in situ cultivars Festivalnaya and Raduga could be marked as plants with higher ecological and physiological potential in arid conditions.
....Concentration of ascorbic acid, depending on the cultivar, varied in the range of 24.99 mg/100 g (‘Michurinka’) up to 47.87 mg/100 g (‘Lany’). Proline content in studied rose cultivars ranged from 9.42mg/100 g (‘Festivalnaya’) to 12.95 mg/100 g (‘Michurinka’).
During vegetation period under hydrothermal stress the total content of phenolic compounds,
particularly flavanols, increased in studied plants, except the cultivar Michurinka. Despite the slower
metabolism of phenolic compounds in the absence of intensive plants` growth and development, increased
synthesis of these compounds may correspond to plant response over stress caused by overheating.
Concentrations of ascorbic acid and proline in this vegetation period varied ambiguous: in the cultivars Festivalnaya and Raduga proline content decreased, whereas ascorbic acid – increased. In the cultivars Lany and Michurinka the opposite trend was noted. The reasons for these differences are specific reaction of plants to the temperature stress, i.e. primary accumulation of specific protective compounds, and physiological features of plant development in this period.
....Phenolic compounds` content in plants cultured in vitro was quite high (1056-1958 mg/100 g). The increased accumulation of phenolic compounds demonstrated high adaptability to the growth conditions and resistance to unfavourable environmental conditions. Thus flavonol concentration in plants cultured in vitro was significantly lower than in intact plants – 23.4-52.0 mg/100g (maximum in ‘Festivalnaya’). Ascorbic acidconcentration in plants in vitro was lower than in intact plants have not been subjected to overheating.
On the contrary, proline content in vitro was higher than in intact plants have not been subjected to thermal stress, up to 10.99-19.62 mg/100 g (maximum in ‘Raduga’). High amount of this compound isprobably, caused with plants` growth and metabolism, as well as nutrients, vitamins and growth regulators level in the culture medium.
Maximum rates of catalase, superoxide dismutase and polyphenoloxidase activity were found in rose cultivars Festivalnaya, Lany, Raduga, cultured in vitro (Table 4).
(2016)  Page(s) 226-232.  
 
The study involved some valuable essential oil rose cultivars, growing on the collection plots of NBG-NSC [Nikita Botanical Garden - National Science Centre]: Raduga, Festivalnaya, Lany, Michurinka.
....The level of water deficit in the first decade of June (average air temperature 21.9°C, minimum relative humidity – 41%) ranged from 15 to 18% in the cultivars Raduga, Festivalnaya and Michurinka; this index was higher in the cultivar Lany (23%). Data obtained in the process of diagnosing the functional state of the leaves through the fluorescence induction of the exposed leaf, demonstrated the absence of photosynthetic apparatus disturbances (relative photosynthetic activity in field studies ranged from 0.66 (‘Michurinka’) to 0.79 a.u. (‘Festivalnaya’). The water holding capacity of leaf tissue was the highest in cultivars Festivalnaya (after 8 hours leaves lose 24-26% of water, after 24 hours – 44-48%) and ‘Raduga’ (28-34% and 39-50%). Minimum water holding capacity was in the cultivars Lany and Michurinka.
....Rapid water loss under wilting of exposed leaves occurred after 4 hours in cultivars Michurinka (21%) and Lany (18%), and after 6 hours of wilting in the cultivars Raduga and Festivalnaya to the level of 20-23%. During the growing season we also observed increase of water holding capacity of leaf tissues: within 24 hours the water loss was 42-48%. Under significant water loss (25-30%) in the leaves of the cultivar Festivalnaya inhibition of photosynthetic function ((Fm-Fst)/Fm=0.75 a.u.) wasn`t observed. In the cultivar Michurinka leaves under the water loss of 15% disturbances in photosystem-2 ((Fm-Fst)/Fm=0.54 a.u.) and at the level of lightharvesting complexes of the photosystem inhibition was noted after 8 hours wilting (water loss 35%).
Thus, on the base of essential oil rose water regime analyses in situ cultivars Festivalnaya and Raduga could be marked as plants with higher ecological and physiological potential in arid conditions.
....Concentration of ascorbic acid, depending on the cultivar, varied in the range of 24.99 mg/100 g (‘Michurinka’) up to 47.87 mg/100 g (‘Lany’). Proline content in studied rose cultivars ranged from 9.42mg/100 g (‘Festivalnaya’) to 12.95 mg/100 g (‘Michurinka’).
During vegetation period under hydrothermal stress the total content of phenolic compounds,
particularly flavanols, increased in studied plants, except the cultivar Michurinka. Despite the slower
metabolism of phenolic compounds in the absence of intensive plants` growth and development, increased
synthesis of these compounds may correspond to plant response over stress caused by overheating.
Concentrations of ascorbic acid and proline in this vegetation period varied ambiguous: in the cultivars Festivalnaya and Raduga proline content decreased, whereas ascorbic acid – increased. In the cultivars Lany and Michurinka the opposite trend was noted. The reasons for these differences are specific reaction of plants to the temperature stress, i.e. primary accumulation of specific protective compounds, and physiological features of plant development in this period.
....Phenolic compounds` content in plants cultured in vitro was quite high (1056-1958 mg/100 g). The increased accumulation of phenolic compounds demonstrated high adaptability to the growth conditions and resistance to unfavourable environmental conditions. Thus flavonol concentration in plants cultured in vitro was significantly lower than in intact plants – 23.4-52.0 mg/100g (maximum in ‘Festivalnaya’). Ascorbic acidconcentration in plants in vitro was lower than in intact plants have not been subjected to overheating.
On the contrary, proline content in vitro was higher than in intact plants have not been subjected to thermal stress, up to 10.99-19.62 mg/100 g (maximum in ‘Raduga’). High amount of this compound isprobably, caused with plants` growth and metabolism, as well as nutrients, vitamins and growth regulators level in the culture medium.
Maximum rates of catalase, superoxide dismutase and polyphenoloxidase activity were found in rose cultivars Festivalnaya, Lany, Raduga, cultured in vitro (Table 4).
 
(2016)  Page(s) 226-232.  
 
The study involved some valuable essential oil rose cultivars, growing on the collection plots of NBG-NSC [Nikita Botanical Garden - National Science Centre]: Raduga, Festivalnaya, Lany, Michurinka.
....The level of water deficit in the first decade of June (average air temperature 21.9°C, minimum relative humidity – 41%) ranged from 15 to 18% in the cultivars Raduga, Festivalnaya and Michurinka; this index was higher in the cultivar Lany (23%). Data obtained in the process of diagnosing the functional state of the leaves through the fluorescence induction of the exposed leaf, demonstrated the absence of photosynthetic apparatus disturbances (relative photosynthetic activity in field studies ranged from 0.66 (‘Michurinka’) to 0.79 a.u. (‘Festivalnaya’). The water holding capacity of leaf tissue was the highest in cultivars Festivalnaya (after 8 hours leaves lose 24-26% of water, after 24 hours – 44-48%) and ‘Raduga’ (28-34% and 39-50%). Minimum water holding capacity was in the cultivars Lany and Michurinka.
....Rapid water loss under wilting of exposed leaves occurred after 4 hours in cultivars Michurinka (21%) and Lany (18%), and after 6 hours of wilting in the cultivars Raduga and Festivalnaya to the level of 20-23%. During the growing season we also observed increase of water holding capacity of leaf tissues: within 24 hours the water loss was 42-48%. Under significant water loss (25-30%) in the leaves of the cultivar Festivalnaya inhibition of photosynthetic function ((Fm-Fst)/Fm=0.75 a.u.) wasn`t observed. In the cultivar Michurinka leaves under the water loss of 15% disturbances in photosystem-2 ((Fm-Fst)/Fm=0.54 a.u.) and at the level of lightharvesting complexes of the photosystem inhibition was noted after 8 hours wilting (water loss 35%).
Thus, on the base of essential oil rose water regime analyses in situ cultivars Festivalnaya and Raduga could be marked as plants with higher ecological and physiological potential in arid conditions.
....Concentration of ascorbic acid, depending on the cultivar, varied in the range of 24.99 mg/100 g (‘Michurinka’) up to 47.87 mg/100 g (‘Lany’). Proline content in studied rose cultivars ranged from 9.42mg/100 g (‘Festivalnaya’) to 12.95 mg/100 g (‘Michurinka’).
During vegetation period under hydrothermal stress the total content of phenolic compounds,
particularly flavanols, increased in studied plants, except the cultivar Michurinka. Despite the slower
metabolism of phenolic compounds in the absence of intensive plants` growth and development, increased
synthesis of these compounds may correspond to plant response over stress caused by overheating.
Concentrations of ascorbic acid and proline in this vegetation period varied ambiguous: in the cultivars Festivalnaya and Raduga proline content decreased, whereas ascorbic acid – increased. In the cultivars Lany and Michurinka the opposite trend was noted. The reasons for these differences are specific reaction of plants to the temperature stress, i.e. primary accumulation of specific protective compounds, and physiological features of plant development in this period.
....Phenolic compounds` content in plants cultured in vitro was quite high (1056-1958 mg/100 g). The increased accumulation of phenolic compounds demonstrated high adaptability to the growth conditions and resistance to unfavourable environmental conditions. Thus flavonol concentration in plants cultured in vitro was significantly lower than in intact plants – 23.4-52.0 mg/100g (maximum in ‘Festivalnaya’). Ascorbic acidconcentration in plants in vitro was lower than in intact plants have not been subjected to overheating.
On the contrary, proline content in vitro was higher than in intact plants have not been subjected to thermal stress, up to 10.99-19.62 mg/100 g (maximum in ‘Raduga’). High amount of this compound isprobably, caused with plants` growth and metabolism, as well as nutrients, vitamins and growth regulators level in the culture medium.
Maximum rates of catalase, superoxide dismutase and polyphenoloxidase activity were found in rose cultivars Festivalnaya, Lany, Raduga, cultured in vitro (Table 4).
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